专利名称：Neural progenitor cell differentiation发明人：Khalil Bitar申请号：US14777335申请日：20140317公开号：US09862924B2公开日：20180109
摘要：Differentiation and stability of neural stem cells can be enhanced by in vitro orin vivo culturing with one or more extracellular matrix (ECM) compositions, such ascollagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of theinvention, adult mammalian enteric neuronal progenitor cells can be induced to
differentiate on various substrates derived from components or combinations of neuralECM compositions. Collagen I and IV supported neuronal differentiation and extensiveglial differentiation individually and in combination. Addition of laminin or heparan sulfateto collagen substrates unexpectedly improved neuronal differentiation, increasingneuron number, branching of neuronal processes, and initiation of neuronal networkformation. In another aspect, neuronal subtype differentiation was affected by varyingECM compositions in hydrogels overlaid on intestinal smooth muscle sheets. The matrixcompositions of the present invention can be used to tissue engineer transplantableinnervated GI smooth muscle constructs to remedy aganglionic disorders.
申请人：WAKE FOREST UNIVERSITY HEALTH SCIENCES
地址：Winston-Salem NC US
国籍：US
代理机构：Pepper Hamilton LLP
代理人：Thomas J. Engellenner,Reza Mollaaghababa
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